It's that time again... Let's talk AR's, so get your coffee, tea, mocha lattes or pre-workout, whatever floats your boat.. Let's have a discussion!
Brought to you by Vision (Team PSL)
Lets's talk AR's (androgen receptors)
Recently I've seen a few questions or concerns regarding AR (androgen receptors) site saturation.. Let me start off by stressing this, it's a myth that has been regurgitated over the years,it simply does NOT exist. In fact AR's do NOT down regulate,there's no such thing as receptor down-regulation (pertaining to AAS usage) in fact with the presence of AAS/Androgens concerning a supraphysiological level they will up-regulate,increasing,and constantly expressing new AR sites THROUGH OUT THE BODY AND TISSUE!.. (However, there is 2-3 culprits that will hinder ones gains when ON cycle, fueling the myth of AR site saturation.."1-Progesterone 2-Mysostatin, 3- and at times E1/E2 estro" but we'll get to that later)
About the AR
The AR gene principals are to provide instructions for creating proteins called "androgen receptors".. As we all know, Andro's are hormones and the first one that comes to mind is? That's right, the king,Testosterone...AR's allow the body to react/respond accordingly to these hormones allowing them to preform their direct action (sexual development,muscle growth and recovery,as well as other strong male sexual characteristics and growth and development)..Receptors are found present throughout the body/tissue..This is where they attach/bind to androgens resulting in AR complex binding to DNA in which regulates the activity of AR genes..DNA is the only thing that can turn the GENE off/on if necessary,NOT AAS!
FYI- AR's belong to a family of genes known as NR (nuclear hormone receptors), which are proteins that are found within cells that are responsible for sensing steroid hormones, and also play a pivotal role with homeostasis, which finds balance in the endocrine!
Why do my gains seem to diminish after 10-14 weeks?
This study below can explain why our gains tend to become stagnate,or slow down when using Test,deca/primo.. ( IMO this is the ideal time to add an oral such as Dbol,drol,Tbol,Winny,or Var, which predominantly work as a non AR mediated mechanism, unlike Testosterone,Deca,Primo..As these agents express differentiation of the satellite cells of the muscle,maturing muscle cells,thus this is independent of ARs)
Mol Cell Endocrinol. 2009 Apr 10;302(1):26-32. doi: 10.1016/j.mce.2008.12.019. Epub 2009 Jan 21.Measurement of myostatin concentrations in human serum: Circulating concentrations in young and older men and effects of testosterone administration.
Abstract
Methodological problems, including binding of myostatin to plasma proteins and cross-reactivity of assay reagents with other proteins, have confounded myostatin measurements. Here we describe development of an accurate assay for measuring myostatin concentrations in humans. Monoclonal antibodies that bind to distinct regions of myostatin served as capture and detector antibodies in a sandwich ELISA that used acid treatment to dissociate myostatin from binding proteins. Serum from myostatin-deficient Belgian Blue cattle was used as matrix and recombinant human myostatin as standard. The quantitative range was 0.15-37.50 ng/mL. Intra- and inter-assay CVs in low, mid, and high range were 4.1%, 4.7%, and 7.2%, and 3.9%, 1.6%, and 5.2%, respectively. Myostatin protein was undetectable in sera of Belgian Blue cattle and myostatin knockout mice. Recovery in spiked sera approximated 100%. ActRIIB-Fc or anti-myostatin antibody MYO-029 had no effect on myostatin measurements when assayed at pH 2.5. Myostatin levels were higher in young than older men (mean+/-S.E.M. 8.0+/-0.3 ng/mL vs. 7.0+/-0.4 ng/mL, P=0.03). In men treated with graded doses of testosterone, myostatin levels were significantly higher on day 56 than baseline in both young and older men; changes in myostatin levels were significantly correlated with changes in total and free testosterone in young men. Myostatin levels were not significantly associated with lean body mass in either young or older men.
CONCLUSION:
Myostatin ELISA has the characteristics of a valid assay: nearly 100% recovery, excellent precision, accuracy, and sufficient sensitivity to enable measurement of myostatin concentrations in men and women.
Measurement of myostatin concentrations in human serum: Circulating concentrations in young and older men and effects of testosterone administration. - PubMed - NCBI
Progestin and AR's
Progesterone has powerful antiadrogenis effect in humans at sufficient levels,capable of decreasing circulating androgens,and estrogen concentraions to castrate levels on both sexes and significantly lowering the expression of the androgen receptor (AR), and the estrogen receptor - (which posses a pivotal in homeostasis/balance and growth)..
read below for continuation-
Brought to you by Vision (Team PSL)
Lets's talk AR's (androgen receptors)
Recently I've seen a few questions or concerns regarding AR (androgen receptors) site saturation.. Let me start off by stressing this, it's a myth that has been regurgitated over the years,it simply does NOT exist. In fact AR's do NOT down regulate,there's no such thing as receptor down-regulation (pertaining to AAS usage) in fact with the presence of AAS/Androgens concerning a supraphysiological level they will up-regulate,increasing,and constantly expressing new AR sites THROUGH OUT THE BODY AND TISSUE!.. (However, there is 2-3 culprits that will hinder ones gains when ON cycle, fueling the myth of AR site saturation.."1-Progesterone 2-Mysostatin, 3- and at times E1/E2 estro" but we'll get to that later)
About the AR
The AR gene principals are to provide instructions for creating proteins called "androgen receptors".. As we all know, Andro's are hormones and the first one that comes to mind is? That's right, the king,Testosterone...AR's allow the body to react/respond accordingly to these hormones allowing them to preform their direct action (sexual development,muscle growth and recovery,as well as other strong male sexual characteristics and growth and development)..Receptors are found present throughout the body/tissue..This is where they attach/bind to androgens resulting in AR complex binding to DNA in which regulates the activity of AR genes..DNA is the only thing that can turn the GENE off/on if necessary,NOT AAS!
FYI- AR's belong to a family of genes known as NR (nuclear hormone receptors), which are proteins that are found within cells that are responsible for sensing steroid hormones, and also play a pivotal role with homeostasis, which finds balance in the endocrine!
Why do my gains seem to diminish after 10-14 weeks?
This study below can explain why our gains tend to become stagnate,or slow down when using Test,deca/primo.. ( IMO this is the ideal time to add an oral such as Dbol,drol,Tbol,Winny,or Var, which predominantly work as a non AR mediated mechanism, unlike Testosterone,Deca,Primo..As these agents express differentiation of the satellite cells of the muscle,maturing muscle cells,thus this is independent of ARs)
Mol Cell Endocrinol. 2009 Apr 10;302(1):26-32. doi: 10.1016/j.mce.2008.12.019. Epub 2009 Jan 21.Measurement of myostatin concentrations in human serum: Circulating concentrations in young and older men and effects of testosterone administration.
Abstract
Methodological problems, including binding of myostatin to plasma proteins and cross-reactivity of assay reagents with other proteins, have confounded myostatin measurements. Here we describe development of an accurate assay for measuring myostatin concentrations in humans. Monoclonal antibodies that bind to distinct regions of myostatin served as capture and detector antibodies in a sandwich ELISA that used acid treatment to dissociate myostatin from binding proteins. Serum from myostatin-deficient Belgian Blue cattle was used as matrix and recombinant human myostatin as standard. The quantitative range was 0.15-37.50 ng/mL. Intra- and inter-assay CVs in low, mid, and high range were 4.1%, 4.7%, and 7.2%, and 3.9%, 1.6%, and 5.2%, respectively. Myostatin protein was undetectable in sera of Belgian Blue cattle and myostatin knockout mice. Recovery in spiked sera approximated 100%. ActRIIB-Fc or anti-myostatin antibody MYO-029 had no effect on myostatin measurements when assayed at pH 2.5. Myostatin levels were higher in young than older men (mean+/-S.E.M. 8.0+/-0.3 ng/mL vs. 7.0+/-0.4 ng/mL, P=0.03). In men treated with graded doses of testosterone, myostatin levels were significantly higher on day 56 than baseline in both young and older men; changes in myostatin levels were significantly correlated with changes in total and free testosterone in young men. Myostatin levels were not significantly associated with lean body mass in either young or older men.
CONCLUSION:
Myostatin ELISA has the characteristics of a valid assay: nearly 100% recovery, excellent precision, accuracy, and sufficient sensitivity to enable measurement of myostatin concentrations in men and women.
Measurement of myostatin concentrations in human serum: Circulating concentrations in young and older men and effects of testosterone administration. - PubMed - NCBI
Progestin and AR's
Progesterone has powerful antiadrogenis effect in humans at sufficient levels,capable of decreasing circulating androgens,and estrogen concentraions to castrate levels on both sexes and significantly lowering the expression of the androgen receptor (AR), and the estrogen receptor - (which posses a pivotal in homeostasis/balance and growth)..
read below for continuation-